To see the effects of any adjustments we make, we can use the Live preview option. We can achieve this by leaving Below threshold to be blank, or alternatively setting it to Unclassified. the channel name), and Below threshold should not have a classification at all. Here, we want Above threshold to be the classification for a ‘positive’ cell (i.e. Next, we then choose which measurement is relevant for the selected channel using the Measurement drop-down list, and adjust the threshold for that measurement with the Threshold slider.Ĭells having measurements with values greater than or equal to the threshold will be assigned the classification selected through the Above threshold drop-down list, and the rest assigned the classification through Below threshold. We should set this to be the first channel we want to use for classification. The Channel filter will be helpful, because it will help us quickly set sensible defaults for the options below. In this case, we can ignore the Object filter (all our detections are cells, so no need to distinguish between them).
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January 2023
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